Food Dye Lab Essay
Summary: The purpose of this lab was to determine the food dyes present in a selected drink, their focus, and then replicate the solution in a 20% margin of problem. The drink we selected was Gatorade Frost Riptide Rush. A spectrometer was used to determine which will food dyes were present in the sampled beverage, red 40 and blue you were determined to be present. The next step was to determine the concentration in the dyes, it was done by testing the absorbance of the reddish 40 and blue 1 dyes in varying concentrations; 100%, 80 percent, 60%, 40%, and twenty percent. The benefits for each color were after that plotted within a graph and a trendline was added. The mountains of the trendlines (y=mx) for red forty and blue 1 had been then used to determine the concentrations, which are found to be 15. 34% (0. 0000138 mol/L) and 14. 12% (0. 0000028 mol/L) respectively. To repeat the color in the original drink a 100mL solution was designed, containing 12-15. 34mL red 40, 18. 12mL blue 1, and 70. 54mL deionized (DI) water. Advantages: This research laboratory was split up into three areas; identifying which dyes exist, determining the concentration of those dyes, and finally to reconstruct the solution within a 20% margin of error. To identify the dyes present a sample in the beverage was tested in a spectrometer, the best absorption rates were found to be at 503. six nm (A=0. 281) and 630. 7 nm (A=0. 270). This kind of lead to the conclusion that the beverage contained red 40 and blue 1 . To determine the focus of the dyes a series of dilutions was well prepared for equally dyes and tested while using spectrometer. This kind of data was converted into point graphs and a trendline was established. The slope in the trendline (y=mx) was then simply multiplied by the Absorbance in the associated color to find the attentiveness. The final objective was to reproduce the drink using the info gathered. To do this a solution was created consisting of 15. 34% red 40 and 14. 12% blue 1 . Experimental Procedure: To complete the test a variety of components and tools was essential. The spectrometer tests required a computer with Logger Expert software, samples of Gatorade Frost Riptide Hurry as well as reddish colored 40 and blue one particular dyes, DALAM water, a spectrometer, cuvettes. In order to prepare the dilutions and duplicated solution 10mL & 100mL graduated cyl, various beakers, a 200mL erlenmeyer flask, and a glass stir rod. To identify the dyes present in the Gatorade, a test was performed while using spectrometer. The first thing was to system the spectrometer and calibrate it utilizing a sample of pure DI water. Following this a cuvette was somewhat filled with an example of Gatorade and inserted into the spectrometer to be examined. The points of highest compression were decided to indicate the presence of red and blue dyes. The initial step to determine the concentration in the dyes previously found was to prepare a group of serial dilutions at 20%/40%/60%/80%/100% dye attention. To prepare the 20% dilution 1mL of red 40/blue 1 was combined with 4mL of PADA water. This method was used to make the remaining dilutions with 2mL/3mL, 3mL/2mL, 4mL/1mL, and 5mL/0mL (mL color / mL DI water). Each of the prior dilutions was tested inside the spectrometer and data was recorded. Using the info obtained two Beer Regulation plots were plotted to get both chemical dyes and trendlines were added. The slope of the trendline was located and accustomed to calculate using the (mol/L) and percent concentrations of reddish 40 and blue 1 in the picked Gatorade. To be able to replicate colour of the Gatorade, a 100mL solution composed of red 45, blue you, and DALAM water. This is accomplished by incorporating 15. 34mL red 45, 14. 12mL blue 1, and 75. 54mL DI water within a 200mL erlenmeyer flask. An example of this answer was in that case transferred to a cuvette to get tested inside the spectrometer. The perfect solution is was examined alongside the information from the Gatorade sample, plus the margin of error was calculated in conclusion how identical the two solutions were.
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